Kinase Browser
Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.
Detailed Locus Information for CNAG_03701T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | 3-phosphoshikimate 1-carboxyvinyltransferase | |||||||||
| Gene Symbol | Unknown | |||||||||
| Kinase Superfamily | P-loop containing nucleoside triphosphate hydrolases | |||||||||
| Kinase Family | Shikimate kinase (AroK) | |||||||||
| KEGG Linkout | KEGG::CNB01990 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSSSSADVLKISILGNESIHVGFHLLPYIFKTVTTTLPSSTYVLITDTNLAAIYLNDLKASFEEATAEADNKARFLVYEVAPGEGAKSRKVKGEIEDWMLDNKCTRDTVILAFGGGVIGDLTGFVAATFMRGVKFVQIPTTLLAMVDSSVGGKTAIDTPHGKNLIGAFWQPSYIFADLAFLTTLPTREVSNGMAEVVKTAAIWKDDDFALLESRSAEISLAASSRPTGAPTAGRFISDRSHAQSLLLQVVSGSIYVKAHIVTIDERETGLRNLVNFGHTIGHAIEAVLTPAMLHGECVSVGIVLEAEVARQLGVLSQVAVGRLTRCLQAYGLPVSLSDRRIAALPASSQLSVDRLLDIMKIDKKNSGPAKKIVLLSRIGKTYEEKASVVADNVIRKVLCEAVTVKAATPSKSPITMATPGSKSISNRALVLAALGKGTCRVRNLLHSDDTAVMMNALVELKGAVFSWEDGGDTIVVEGGGGILSTPAKGKELYLGNAGTASRFLTTVCAMVSGSASSERSTIITGNARMKQRPIGPLVDALTANGAKVKYLESTGCLPLDISTDGFRGGHIQLAASVSSQYVSSILLCAPYAAEQVTLELTGGQVISQPYIDMTIAMMEQFGATVERQKDEQGNLLDVYVIPKCTYVNPSEYSVESDASSATYPLAIAAITGTTCTISNIGSSSLQGDARFAKEVLEPMGCIVEQTLTSTKVTGPPVGTLRALGNVDMEPMTDAFLTASVLAAVAVKPCLPERKVEGLPETASRIYGIANQRVKECNRIQAMRDQLAKFGVETDEFDDGIIIFGKPEASLFRGASIHCYDDHRVAMAFAVLSCIIDETIIEEKRCVEKTWPNFWDDLQNKIGVAVEGVELETHNQASTSAKPVSPIDQSQSDRPIFLIGMRGAGKTYVGRMAAEILSGQFTDADDVFAQETRQSVSEFVAANGWDEFRKKETEILSKFVEEHRGNHVIALGGGIVETEAARETLKAHVAKGGHVVHVTRALEDIEAYLDSIGNTAVRPNWGETFADVFKRREPWYQACSSHEFYNVLEAVGGQTHEEHTKAMRAECGRFFKFITGRESNRPRLSAGNPTSFLSLTFPDITPALVHLDELTEGADAVEFRIDLLNTTGHAPTSPASPPISFVAKQLASLRLATTLPIVFSVRSKDQGGMVPSDNAEAYGALVRLGLRCACEYVDLEVCWPEQLLDSIVQLKRETHIIASWHDWTGDMAWDGEEMKAKHVLCEKYGDVAKIVGTAKSGLDNAKLAIFVGEVQSHPGAKPLLAINMGAAGQLSRVLNPILTPVTHDALPSRAAPGQLTAREILQARALTGSLPAKKFVLFGSPIAHSVSPLLHNTGFATLGLPHNYGLHESEKVDQGVLEMIRSPEFGGASVTIPLKLDIIPHLDSVSEDAKIIGAVNTVIPRGGKLYGENTDWQAIRQAAAQNLDDDVLSDGSSTALVIGAGGTCRAAIYAMHKLRFKTIYLFNRTPENAAKVKASFPESYNIAIVTSLSSLPEAPVVVVSTVPGNSLTLDTFSQGIYLPSEVFSRPKGVAIDLAYKPHMTALLQAAEKKEGWKALPGVEILCLQGFKQFEEWTGKRAPEKKMRKAVLDKYFA | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 22312 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 13396 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 12917 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 14542 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 16254 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 13737 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_03701 5' flanking region primer 1 | AGCGTATTCTTCAGGGCTC |
| L2 | CNAG_03701 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACAAGAAGGGAGAGTGGTTGTGACGG |
| R1 | CNAG_03701 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTGAAGTGTTTTCCCGTCCC |
| R2 | CNAG_03701 3' flanking region primer 2 | TAAAGGAGTGTTGGACCCC |
| SO | CNAG_03701 diagnostic screening primer, pairing with B79 | ACAAACCTCACTGTGCCTC |
| PO | CNAG_03701 Southern blot probe primer | CAATACCGACTGAGACACACTC |
| STM | NAT#125 STM primer | CGCTACAGCCAGCGCGCGCAAGCG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |