Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03796T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationNAK protein kinase
Gene SymbolENV7
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNB02900 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
CAMK-4.60.000000000000038
Microbial Kinome Result
PredictionScoreE-value
ePK159.21.5e-47
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K08856 KEGG 6.7e-138 459.6
Amino Acid SequenceMSRIPDTMDSDAAAIALDRLKFYARDALLTITQCICKPDATLKINGRSYKIEKLLGEGGFSFVYLIRDLSSDRLYALKKILVTSGQEGVKEAMREVEAYRRFRHPNIIRILDSAVVQDESGDGKIIYLFLPYYSKGNLQDAMANASVTGQRIPERKLLELFHGTCLAVRAMHQYHLPNISASYPPTREDEPLVGETVFDHDEELTEEDQGELVPYAHRDIKPANIMISDEDEPILMDFGSTIKARIAIETRQQALLEQDIAGEHSSMPYRAPELFDVKTGKTLDEKCDIWSLGCTLYAVAYGHSPFEVDGQSIAMAVGSGRYRHLTGYSQDFVQLIDSMLIVDPENRPDIQKVIEMTENILRKT
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 3640
B YPD 30°C, concentration: 5x10^7 cells/ml 5383
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 5662
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 5242
E YPD 37°C, concentration: 5x10^7 cells/ml 4088
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 2817
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03796 5' flanking region primer 1AGGTCGGAAGATTTTGCG
L2CNAG_03796 5' flanking region primer 2 TCACTGGCCGTCGTTTTACTAGGGTCGTTTGTGTTATCC
R1CNAG_03796 3' flanking region primer 1 CATGGTCATAGCTGTTTCCTGCTTTTGGCTTTGGGTCAG
R2CNAG_03796 3' flanking region primer 2 TGAGCAGTAGTGTATTGGGTG
SOCNAG_03796 diagnostic screening primer, pairing with B79AATCTCCTCTTGGGCTCAG
PO1CNAG_03796 Southern blot probe primer 1AAAAGAGACATCGGCTACG
PO2CNAG_03796 Southern blot probe primer 2ATTTGGATGCCGAAAGCG
STMNAT#227 STM primerTCGTGGTTTAGAGGGAGCGC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_03796T0
Mating Data
Growth phenotype for CNAG_03796T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_03796T0
Urease
Urease phenotype for CNAG_03796T0
Melanin
Melanin phenotype for CNAG_03796T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_03796T0
Oxidative Stress
Oxidative stress phenotype for CNAG_03796T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_03796T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_03796T0
ER Stress
ER stress phenotype for CNAG_03796T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_03796T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_03796T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_03796T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_03796T0