Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03821T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationphosphatidylinositol 3-kinase
Gene SymbolUnknown
Kinase SuperfamilyARM repeat
Kinase FamilyPhoshoinositide 3-kinase (PI3K) helical domain
KEGG Linkout KEGG::CNB03120 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
PIKK-147.10.0000068
Microbial Kinome Result
PredictionScoreE-value
PI3K_Sugen228.82.8e-68
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K00914 KEGG 0 1021.3
Pfam Annotation
Pfam AccessionE-valueScore
PF00454 Pfam 1.2e-56 184.4
PF00613 Pfam 1.8e-50 168.9
PF00792 Pfam 4.8e-36 121.8
Amino Acid SequenceMQPGPSKDRDYSFARLCHLTPHLALKITSLQGSLPKHPFSRALHSPALAHQGLQQQTPSDLYVTCQLWADGKQYSLPFRTAHKDFPRGYTWNSVVIFPITYPSLLLSSQIAFTIWDVQGSGKAIPVGGTTMSLFNSKRTLKRGQQRLYVHRNVQADPSLNTTTPSEFPDEKEDEMGRLERLVKDFERGDILKIDWLDRLTFRQIEKAHSAEAEKSDNLYLYVDLPKFDFPVVFSEQESLITLPPQPVIHPPSQTSQPTSALPPNLLSNDPHLWKTYDPEAWRENPVEIKHRKLLRSQRLGDEGRDLKPGPADRDRLNEIFRLPPTASLSAVDKDLLWKFRFSLFRSPRSLTKFLKCITWSDPVEAKQAVEKLLPLWGQEVGMDDALELLGPNFTHKKVRAFAVKRLERAEDDELLLYLLQLVQALKFEHKSSLDVQRGHRSHRKRERELASQDEQSSGLSQFLINRSVANPILGTSFHWYLMIECDNRSSVGKMYAQVAFNFMKKLSETPKGQAQRDILRRQGELVQILSTRAKDIRASKDSRSKKIEKLKAYLSDSKHGLASLPEPLPLPLNANIFVTSVVAEKSSIFKSNLLPLLIWFETIDSTRPTDDDSPDAVVSITPDYPIIFKNGDDLRQDQLVIQLFTLMDRLLRKENLDLRLSPYSVLATSTTEGMIQFVPSKSVASIMAEHGSLQNYLRIEHADDGALGSYGIEASVMDTFVRSCAGYSVLTYVLGVGDRHLDNLMLAPDGHFFHVDFGYILGRDPKPYPPPVKVCKEMVDAMGGPGSAHYGRFQSLCYTAFIGLRKNANLILNLVALMVDAGIQDIQLEPDKAVWKVQEKFMLDLSEEDAIKQFEVLLNDTSYLTAVFDRIHDWAQYLRD
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 4252
B YPD 30°C, concentration: 5x10^7 cells/ml 3980
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 4346
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 3252
E YPD 37°C, concentration: 5x10^7 cells/ml 3315
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 3900
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03821 5' flanking region primer 1GGGTCATTTTCACCGAATC
L2CNAG_03821 5' flanking region primer 2TCACTGGCCGTCGTTTTACCTTTGTGTGCCGTTCTAAAC
R1CNAG_03821 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGGCCAGATGGTCATTTCTTC
R2CNAG_03821 3' flanking region primer 2GGAAATAGAAACAGCGGTG
SOCNAG_03821 diagnostic screening primer, pairing with B79ACCAGGTCTTCCTCCATTG
POCNAG_03821 Southern blot probe primerTGAGAGATTCTTGTTCCGAG
STMNAT#177 STM primerCACCAACTCCCCATCTCCAT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG