Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_04108T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationpyruvate dehydrogenase kinase
Gene SymbolPKP2
Kinase SuperfamilyATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
Kinase Familyalpha-ketoacid dehydrogenase kinase, C-terminal domain
KEGG Linkout KEGG::CNI04400 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
PDHK283.71.5e-84
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K00898 KEGG 9.5e-169 561.5
Pfam Annotation
Pfam AccessionE-valueScore
PF10436 Pfam 2.2e-56 188.2
Amino Acid SequenceMSKFRISSALWDKIHHFASFPQTGVSLQQMILFGQNPSQGTLLKASQFLSEELPIRLSHRVVELEGLPDGLSKMPSINTVKEWYAQSFDELVSFPRPRLKPELEDILRIPPAPTQPFPSATPNPSLDPLMREGPVGSGFVSERHIGTGGNGIGVGKRLRIPIERRYFSPPPPTIIYPPEVHDYNEKFTQLLQVIKHRHDPTVTTVAQGVLEWKRTQKTSVIGTPIQEFLDRFYMSRIGIRFLIGQHIALNTLPPHPDYVGIICTRANIHDICQEAIDNARYVCEEHYALFKSPNIKLVCPPNLTFPYIPGHLSHICFELLKNSLRAVVERYGVDNDDEYPPIKVVVVEGREDITIKISDEGGGIPRSAIPHIWTYLYTTMSDEGLEDTIQGSDFKAPMAGFGYGLPLSRLYARFFGGDLRLISMDGYGTDVYISLNKLSSSREPLQ
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 5533
B YPD 30°C, concentration: 5x10^7 cells/ml 4353
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 4486
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 3022
E YPD 37°C, concentration: 5x10^7 cells/ml 2382
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 3010
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_04108 5' flanking region primer 1AAAAGAGGAGGGAGAAGGG
L2CNAG_04108 5' flanking region primer 2TCACTGGCCGTCGTTTTACTGAAGTATCCACACACCCC
R1CNAG_04108 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCGTCTTTGAGTTAGGTGCTG
R2CNAG_04108 3' flanking region primer 2TGATTGGGGAAGCGTTAG
SOCNAG_04108 diagnostic screening primer, pairing with B79TGTCGGTTTTGTGGTTCC
POCNAG_04108 Southern blot probe primerTTAGCCTCTTGCCAACTCC
STMNAT#295 STM primerACACCTACATCAAACCCTCCC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_04108T0
Mating Data
Growth phenotype for CNAG_04108T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_04108T0
Urease
Urease phenotype for CNAG_04108T0
Melanin
Melanin phenotype for CNAG_04108T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_04108T0
Oxidative Stress
Oxidative stress phenotype for CNAG_04108T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_04108T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_04108T0
ER Stress
ER stress phenotype for CNAG_04108T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_04108T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_04108T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_04108T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_04108T0