Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_04282T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationCMGC/MAPK protein kinase
Gene SymbolMPK2
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNI02670 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
CMGC308.74.4e-92
Microbial Kinome Result
PredictionScoreE-value
ePK221.35e-66
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K04464 KEGG 5.5e-174 578.2
Amino Acid SequenceMTSPSTQPMIPRSQPPRPAHTQSSPAINTTGIKPSGSPAVIKRAPSVPVNPSTAAASESLSSLLKSENAAEAVPRPSSSTEPNDLPRPIEPAPVPPITGLPNSSSSSSNHRHGSSSSVSRGPSGTGTQTPATTALGAPIGPTPPQTGASKMAAAAKRGLDGSAGNHVKVYEEGHPLNEETLKQRGYQTLNVLNHPFHLSDRWKLLRPLGQGAYGLVIQVQDAETEIPIAVKCVTRVFDKVILARRALREITLLRHFGEHDNLTGLVDMDNVWEGYNEIYLYMEPMEADLHQIIRSGQPLGNAHIQFFIYQLLRGMKYIHSANVIHRDLKPGNLLVNSDCELKICDFGLARGFNPVMGEEPQGEEGKLTEYVATRWYRAPEIMLSNRRYTTAIDVWSIGCILAELLGLKPMFKGKDYIEQMTLILETLGTPDEETMTQVASEKALTFLKTLPTYEKKDLKSIFPDADPLAVDLTDKLLEFDPTRRIDVPTALTHAYVERYHDPEDEPSCEKIFDKWKEVESLGTIEELKEAITREIQGFREEVRTAAEEDDEDEEWAEGDYLEDGKVFHDSPVPPLASTLEKVAYPDHIAPSSSMPPSAVRLAPAIHQSPKDSKSLHVKSAHLPSVPSSANSSKPSSTAGSAPRTREHSPTTTFTTPSEDYFTGQHGSGGPNGRASRRSSTHSTSGRRPASFLFSPFGAGMTQIVSTPNTHPRSHAQASPEPTTVDKTPRDRRSSGIWRTRSRAQSQSGNLVLERLSSLEINDHKDGEKNRIDALHAVVGLGGDGEVPPMTVSPSDAPPSEVPKSFGY
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 1294
B YPD 30°C, concentration: 5x10^7 cells/ml 996
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 1054
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 992
E YPD 37°C, concentration: 5x10^7 cells/ml 995
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 1611
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_04282 5' flanking region primer 1ATGGCAGCAAGCGTAACTC
L2CNAG_04282 5' flanking region primer 2TCACTGGCCGTCGTTTTACGTTTTATGCCCGTTGTGTTG
R1CNAG_04282 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCCCAAAGTCAGTCTGGTAACC
R2CNAG_04282 3' flanking region primer 2ATACATCTTCGTAGCCCCG
SOCNAG_04282 diagnostic screening primer, pairing with B79TCCAAATAGACCAAGCCC
POCNAG_04282 Southern blot probe primerCGTTGAGTGTTTGGTAGCC
STMNAT#102 STM primerCCATAGCGATATCTACCCCAATCT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_04282T0
Mating Data
Growth phenotype for CNAG_04282T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_04282T0
Urease
Urease phenotype for CNAG_04282T0
Melanin
Melanin phenotype for CNAG_04282T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_04282T0
Oxidative Stress
Oxidative stress phenotype for CNAG_04282T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_04282T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_04282T0
ER Stress
ER stress phenotype for CNAG_04282T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_04282T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_04282T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_04282T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_04282T0