Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_04433T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationCMGC/DYRK/DYRK2 protein kinase
Gene SymbolYAK103
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNI01240 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
CMGC330.61.1e-98
Microbial Kinome Result
PredictionScoreE-value
ePK203.61.1e-60
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K18669 KEGG 1.2e-199 649.6
Amino Acid SequenceMISRPAPMSSPEPPPCFPPQQPQASPGYPDSALKPASPAPCLPRVPAPPPPAHPTHVPPPADQRDRLPGSSSIASMSSSPRGRRAPAPAALDLGAGPGGSKYAGVGLGLGVGHEAEKRRVVTEPSQRISRPLPHPPSHHEAPCSIHPYPTSLASAPYSAQPPSSAQRHPASIDRRNLVGVGELSTPRWPGSGFHSRTPSMPHNMASPSSRLPPLPQTPTIQQGDYQRSTSATQSPPRPVHTTSPATQPTREVLTAPNTAAHDGSLYSLASLAQFNFDSTMEASLAASLATLTTSPKVDSPKVNSPKMNSPKSPVLPQLDSILPPSPGSARSRIYARRQERANAAASISQPGPSHQSISHSTSQPQSISRPQPPAESTFSPRRDSRKTPPNPSRHNPSHDILKQFAVKDFSHLPPSPSSASINHFLRASGSTQTISSGTPPASASASNTYFPSKGVQREDSQKSQKSQKQQRTTPKPRELDPNVDEALRKLDGLTSTPGKNRTRGKSSGGPSAASSRPGTPVAESKSRSEGKIPSKPSIGSFKDATGSPLNNWIDLTEDVPAIPIPRPRIPPNHESSSSASVTDTPNSHDSQSLPTTATTMSSADLANNNKPRRASAGSDTFVPQNVDQQPEETEKSIVPPVPPLPQVYVNKKQTMPVQSSAMPVQSSAMQPPNYVPMREPASPISSPSESRSPSSTTAPVPVPVPPKMHKKWSFSSALNLKSATSPASSVDEMTMATPQSPQTPWSEIHRAELFSPSSSVQGQDSGGDSGPQGATPLAPPPPPPPPSSKTSNVNKRLTPSSIPFFRRSSSSSFQSKASQPAAPVPETPKQTEKTAAATVVPSSQSSRKSVLGMHFPSMLRGSSSKRGLAQQTGQSQVSAKEKEGIEPTSAPSATSGWTGRKRGKTLSISGDLPKPFPGLKHQSSADSSFGTRGSAVSSHSNTTISGSNTYDRLPAIMGSPARPPEGPRYSNSPRNLPSTTPTKIPRMTQRPAPSPATVTAHSGMPPPPFPTIRKISNTVSGAAGEQQQQQHRGGGGPPVSEFGMVDGIPNIPTTPRLTTSGAHRAHLLAPMSARQETRRVNNNRPYDPPLVRKEAYNSAVPPSRRQLPQPPPSSSTVTNMTLSASAKRASREFKNNKRRESKDGQDGSSSGKNSPVKPSKSMHSSMNVPSSSRLPSSFSAGAPGSNYRKLSLADSSPSMSPADDDEVSGDAEMEAYIKRRRERAAVNKKDDLSDVTEFPRDITPVEPLSQRAFITRNLAKMSDAEKKEVLDFDHIYYSPTPGTIRRPSQPGGAMFNHGYDDERGDYLVVEGDHLCYRYEVVGVLGKGSFGQVVQCRDHKTGKSVAVKIIRNKKRFHAQALVEVKILQQLIEWDPEDKHFMVRMTDSFSFRNHLCIVTELLSINLYELIKANHFAGFSTVLIRRFTTQMLGSLQLMRSHRIVHCDLKPENILLCHPSKSAIKVIDFGSSCLETEKVYTYIQSRFYRSPEVILGMNYAMAIDMWSLGCILAELYTGVPIFPGENEHEQLACIMEVLGVPDRYLIEKASRRKNFFDATGAPRPFVNAKGRRRRPGSKTLAGVLKCDDELFVDFIARCLTWDPDKRLKPQPAMRHPWILAGRRRYAPTPSRDEKRAATERSSRLFGSSHTSTRISSKNLSELSTPSMKDKDKSKFLISSPVPLAPAKHPYHASTASTSRIGQAMSSSRLAHQTSARSGSLTTSKLSIG
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 11463
B YPD 30°C, concentration: 5x10^7 cells/ml 6485
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 8057
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 5328
E YPD 37°C, concentration: 5x10^7 cells/ml 7532
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 10823
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_04433 5' flanking region primer 1AGCCTGTGAGTTGTGCGTTG
L2CNAG_04433 5' flanking region primer 2TCACTGGCCGTCGTTTTACGGTTTTCCTGCTATCACGC
R1CNAG_04433 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGGACCTCAAAACTCAGCATTG
R2CNAG_04433 3' flanking region primer 2AAGAAACCTCTCCATTCCC
SOCNAG_04433 diagnostic screening primer, pairing with B79AATACCTTGTTGGCGAGAC
POCNAG_04433 Southern blot probe primerCATCAGGAGGTTTACCACC
STMNAT#231 STM primerGAGAGATCCCAACATCACGC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_04433T0
Mating Data
Growth phenotype for CNAG_04433T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_04433T0
Urease
Urease phenotype for CNAG_04433T0
Melanin
Melanin phenotype for CNAG_04433T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_04433T0
Oxidative Stress
Oxidative stress phenotype for CNAG_04433T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_04433T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_04433T0
ER Stress
ER stress phenotype for CNAG_04433T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_04433T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_04433T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_04433T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_04433T0