Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_04631T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationribitol kinase
Gene SymbolRIK1
Kinase SuperfamilyActin-like ATPase domain
Kinase FamilyGlycerol kinase
KEGG Linkout KEGG::CNJ00680 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction N/A
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K00875 KEGG 7.4e-214 710.6
Pfam Annotation
Pfam AccessionE-valueScore
PF02782 Pfam 2.1e-52 176
Amino Acid SequenceMSDMEYFIGFDVGTGSGRACLVDRNGNLIAEHAEPTLTHRSPTDARIFEQSTGNIWKSLSICSKKILAESGVKPEQVKGLGFDATCSLAVVDKQGKPVSISRAGQTEENEKDANLGLEGEWNVILWADHRAEEEAEKINSTGEGVLGFVGKTMSLEMEIPKTLWLSKHMAQEKFKQCMFFDLPDFLTYNATSDLARSTCSLACKCSFVPIGATMTHDCDGGKEEVSTQGWSARFFEKIGLHDLVKEDFASLGGIPGKNGLVLTAGQPVGKGLSKRAAEELGLLEGTAVGSGVIDAYAGWIGTVAAAAGEDQAQPTLEDASTRLAAIAGTSTCHIAQSKDGILVPGVWGPYRDAVFPNLWMNEGGQSSTGQLIDFMMQSHPAYPKLLELSKSSGKSTFELLAERLETLQAEKAAKTLTHLTKDLHFYPDLHGNRSPLADPRMKGMITGLVLDDTLNDLAAKFNVTLEAIALQTRHIVDEMNAKGHKIDSIYMSGSQAKNGPLMRLLATVLQMPVIIPPQPSAAVVLGAAMLGRYAYDLTTERQGKPISSQQEAEVAKEKDGWRLWDVMVQMTRPGKRIEPRNDKFGISEKRLLDVKYKIFREAVEVQRRWRQMIADEVKEEE
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 3215
B YPD 30°C, concentration: 5x10^7 cells/ml 1710
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 1906
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 1702
E YPD 37°C, concentration: 5x10^7 cells/ml 990
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 4265
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_04631 5' flanking region primer 1TCATCAGTTTCGTCCAGC
L2CNAG_04631 5' flanking region primer 2TCACTGGCCGTCGTTTTACATAACGGGATTGGGGTTG
R1CNAG_04631 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTTGCTGATGAGGTCAAGG
R2CNAG_04631 3' flanking region primer 2ATCTCACTGCCCTATTCCC
SOCNAG_04631 diagnostic screening primer, pairing with B79TTCCACTCCTTCTCCCTCTG
POCNAG_04631 Southern blot probe primerCAGGAAGGCTAAAACCACAG
STMNAT#150 STM primerACATACACCCCCATCCCCCC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_04631T0
Mating Data
Growth phenotype for CNAG_04631T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_04631T0
Urease
Urease phenotype for CNAG_04631T0
Melanin
Melanin phenotype for CNAG_04631T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_04631T0
Oxidative Stress
Oxidative stress phenotype for CNAG_04631T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_04631T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_04631T0
ER Stress
ER stress phenotype for CNAG_04631T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_04631T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_04631T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_04631T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_04631T0