Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_04821T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationPAB-dependent poly(A)-specific ribonuclease subunit PAN3
Gene SymbolPAN3
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNJ02470 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Microbial Kinome Result
PredictionScoreE-value
ePK25.80.0000000015
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K12572 KEGG 8.1e-204 678.2
Pfam Annotation
Pfam AccessionE-valueScore
PF18101 Pfam 1.7e-63 211.1
Amino Acid SequenceMLPPPKSAAVQIVRPPSPSSEKAKEKEKKHSPEKRETPQRICRNVMIYGYCKYQDQGCIYYHPPAGADPSTPQESSPATHAPTPLAGTPAREKPTLSIEHLAAPVFVPKGLDSSSPRVATPSAPTPSAPTPPVWPSLPSTGLLPRQDVHVSARPSHAQLSATASPMAYDDPSHIALSAAHAHAQAQALTHSMLDPHAHAPPVDQSMYLPPRQPLDYNLYAAPLPSIGGNPLYPAHPHAFFVNDDLRRSIQAKQEAVYVGANGASAPGLPQELGVYHSLVPLPLPAPTAQRLPTQSQPSKVYGLPSPVYRATSEVDGNTYCLRRVEGFKLVNQLAFASMDTWRRMRHPNIVGLKEAFTTKTFGDNSLIMVYDYHPLSTTLYDEYLSPNPPEPSPASLLAGNQPPKRRSSPPERILWSYVTQIANALKAIHSSGLAVRNLDASKILLTGKNRIRLNGCGVWDVLAFDNKTPVQAFQQEDLLSFGQLVISLTCDFFQPTLPLSLPLEHISRHYSSDLSNLILYLISKPAQGQVKSIDEVVKMMGPRILNELDAVQSYADVLENELGAEVENGRIVRLLTKLGFINERAEFELDPRWSDTGDRYILKLFRDYVFHSVGVDGKPILDLSHVLVCLNKLDAGLDERVMLVSRDDQSCLVVSYREIKHCIEAAFNELKNAGNNHRVHR
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 5431
B YPD 30°C, concentration: 5x10^7 cells/ml 7595
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 7557
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 5910
E YPD 37°C, concentration: 5x10^7 cells/ml 6841
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 5236
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_04821 5' flanking region primer 1CTCTTACAGACGGTTCTTTAGG
L2CNAG_04821 5' flanking region primer 2TCACTGGCCGTCGTTTTACTCTCCTTTGCCTTCTCCGAG
R1CNAG_04821 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGAGAATGCGGGCAATAACC
R2CNAG_04821 3' flanking region primer 2GCCAAAAAGCAAAAAGTGGAGC
SOCNAG_04821 diagnostic screening primer, pairing with B79GCAGGAAGAACAAGGTGTC
POCNAG_04821 Southern blot probe primerGGAACGAGAGAGTGATACACG
STMNAT#204 STM primerGATCTCTCGCGCTTGGGGGA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_04821T0
Mating Data
Growth phenotype for CNAG_04821T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_04821T0
Urease
Urease phenotype for CNAG_04821T0
Melanin
Melanin phenotype for CNAG_04821T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_04821T0
Oxidative Stress
Oxidative stress phenotype for CNAG_04821T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_04821T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_04821T0
ER Stress
ER stress phenotype for CNAG_04821T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_04821T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_04821T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_04821T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_04821T0