Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_00745T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationHAL protein kinase
Gene SymbolHRK1/NPH1
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNA07240 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
CAMK26.16e-17
Microbial Kinome Result
PredictionScoreE-value
ePK173.01.7e-51
Functional Annotation N/A
Amino Acid SequenceMLSSNPSIPLSSIDFADNEGRRLSLVTAEGRKMLTPHDGLPSPGNLSPVAGGTPTENGSDFGEGADIADERSRQMRYSDDDESTLNTVDVEQPVGAMHHRLSKVPSRGGIWHPDPRWDESATSRRGSPAARPIDGEQFFAAHNKTFPPNLAFTPAVATPSSTSLKTTSPAPEAQEPQPRAQTAPSSPHQSPTATRQPSKHHHPLHDLRRFLNHTLGGRDKHANDHHHETSGPSTPGSPSMNAATPAIQRRGSGFSGLAGAGGGMTNAQATPAHSRHDKDHGAHTTHLMGFIRHHHRDNDGEKSHSSLASFFGHGDKKDKKKREKGHTPVESAAGSAAPSRTQTLQMTGAESPRSAAGSPRMTPADASGYATPKNASEYPGVPHPVVALTHPSLHEATHAALSKKYGKWGKVLGSGAGGTVRLIKGSSKTGGTLYAVKEFRPRRQGETEKEYQRKVTAEFCVGVTLRHVNVIETVDIVNDHNHFYEIMEYAPYDLFSVVMSGKMSRPEIYCVFRQIVDGVNYLHSMGLAHRDLKLDNCVMTSENIVKLIDFGTATVFHYPGKHQIPATGVVGSDPYLAPEVLSKSQYDPRLTDVWSCAIIFMCMILRRFPWKIPDYKTDMSFRLYVNTHPELCIKPPVPTPAPSLANGNEGGHTKAGGPHGLLPDGSSSLPFRSGSNISGETAVDTHRGSPDSPPRNGHLQGSPTSSSSGEAQLAQLHLRDDNNEKHKDLSDLTFPRRSDSIVSVPASRYATKGGLAPAQPKRQGTLPPSRLAVTEEHRGRNRAVSSPTSQPSTPALDHRGNPINAAVAGSAGTEKKESTPERRAETQAKRERAASISSTRTFKSGGAESIFRLLPRESRSAILRMLAVEPSIRCTLSDLLIGSGKDKMMCPCGSEDCSGSVAQPPSEITGISPNEEDDGDEWVKNIDCCSHHVGRPSNHQHIKVVPEEKPKRKLFH
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 21496
B YPD 30°C, concentration: 5x10^7 cells/ml 7711
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 9149
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 8869
E YPD 37°C, concentration: 5x10^7 cells/ml 6783
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 18489
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_00745 5' flanking region primer 1GCAAAAATGGGGAAGATAGG
L2CNAG_00745 5' flanking region primer 2TCACTGGCCGTCGTTTTACTTCCCCAAAATCACTCCC
R1CNAG_00745 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGGAGATGAGTGGGTGAAG
R2CNAG_00745 3' flanking region primer 2TGTGTCAGACCTGTTATCGTTC
SOCNAG_00745 diagnostic screening primer, pairing withCTCAACCACTCTCTTACGGA
POCNAG_00745 Southern blot probe primerCGAGGTTAGGAGGAAAGGTC
STMNAT#210 STM primerCTAGAGCCCGCCACAACGCT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_00745T0
Mating Data
Growth phenotype for CNAG_00745T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_00745T0
Urease
Urease phenotype for CNAG_00745T0
Melanin
Melanin phenotype for CNAG_00745T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_00745T0
Oxidative Stress
Oxidative stress phenotype for CNAG_00745T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_00745T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_00745T0
ER Stress
ER stress phenotype for CNAG_00745T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_00745T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_00745T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_00745T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_00745T0