Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_05104T1
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationCAMK protein kinase
Gene SymbolFRK101
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNL05170 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
CAMK11.50.0000000000000043
Microbial Kinome Result
PredictionScoreE-value
ePK145.61.1e-43
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K08811 KEGG 2.1e-50 170.9
Amino Acid SequenceMDNTFPTNVLGFYLDCDEYEDPQNNEGPTQPSLGLQNLDRNIVLADYSEFLYSAPSNWLPIWPDFTCCYSEHDGHPCYVFDSDPSLRACGPYDCHDYKTFGVISVGAHGIIHRATDMKRNEMVAIKVTKEYHGSDTGDTHMRWQLWKRSFAAREVAMLEVCDHPHIVKPRDIVESPDGKLGLVIDYFPGGDLQIYIKTYGSLSEFDAQYTFTQLLSAVTHLQDRHMVHHDIKPENILIDLGGNVVLSDFGVAHIFDESFVPALPICGTIVYAEPELVVQKTRYSPFKWDIWSLGIILFFLLTGKTPYIGDK
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 50
B YPD 30°C, concentration: 5x10^7 cells/ml 19
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 15
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 22
E YPD 37°C, concentration: 5x10^7 cells/ml 78
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 129
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_05104 5' flanking region primer 1GCTTTTTGACGAGACAACTG
L2CNAG_05104 5' flanking region primer 2TCACTGGCCGTCGTTTTACGATAAAACCCGAGGACATTC
R1CNAG_05104 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCGTTGCTTCCGTATCTGTTG
R2CNAG_05104 3' flanking region primer 2AGCAAGTGAAAGAAGGGC
SOCNAG_05104 diagnostic screening primer, pairing with B79TATCAGGGCTTGGGTGTAG
POCNAG_05104 Southern blot probe primerAGATGGGAAGCCAGTTAGAC
STMNAT#282 STM primerTCTCTATAGCAAAACCAATC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_05104T1
Mating Data
Growth phenotype for CNAG_05104T1
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_05104T1
Urease
Urease phenotype for CNAG_05104T1
Melanin
Melanin phenotype for CNAG_05104T1
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_05104T1
Oxidative Stress
Oxidative stress phenotype for CNAG_05104T1
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_05104T1
Cell Wall Stress
Cell wall stress phenotype for CNAG_05104T1
ER Stress
ER stress phenotype for CNAG_05104T1
Genotoxic Stress
Genotoxic stress phenotype for CNAG_05104T1
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_05104T1
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_05104T1
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_05104T1