Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_05771T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationserine/threonine-protein kinase TEL1
Gene SymbolTEL1
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyPhoshoinositide 3-kinase (PI3K), catalytic domain
KEGG Linkout KEGG::CNF02070 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
PIKK196.62.4e-58
Microbial Kinome Result
PredictionScoreE-value
PI3K_Sugen109.91.2e-32
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K04728 KEGG 0 1387
Pfam Annotation
Pfam AccessionE-valueScore
PF00454 Pfam 9.2e-51 170.8
PF11640 Pfam 1.4e-30 102.5
Amino Acid SequenceMDSVSGLHAALTLCASESARDRGRAHALLPAIFANPENLLVFHAAAAKHGGAPWLALFHCLFRAVAVEKQAVLRSSTRNAQPAERLSHAIRLVRLVAEQAVHLIARKPLIALLTHMRHQLVLSPRIFAPALLDYSKAINRLLSYPPHLESLDAHTWLALMSISFAAILGDDVLSDDQMDEQDILDVARELALLDTEKKAPPPPPHRPPVTVNTSSLVQIIPALLCSTVSPVVPPTMKSGDTLTAGQKVGLGIVLKIRRFFIMYPHVTIYHLHLLKALNTVLSEMELNCRDLFLLGSVKIFPHLVTLWAAPERDRDRRIPEQIAIAIHKILPHVVHKMSAGVQGAQDVRENVERLMEMLSKESTMSRGIEPLDLASTRLKTIAKGRHHAAACPFETRSFSAGHHFTVDHALSWTAIETYCDSCVYLYQTHPFSITTPSRSGTLSKRRKVENALASLISAIGMGKPESRLVALQTLVFVIDKHWSKLANDIHSDIRQTLISLLRDDNEVLQSWAYIALSTIILSSHTTGAQENDQSQQDDWNQVWSFALRKCHLPGTCRSASHAAAILLQFDKLDSTSTIKGIQSILSSIAVQGPPTPHDSVCALYSVALEAARSDIQLYSLDLEEQVCSWLEKTLAGEKFERDKMDQRLDQATPGDILRLFSAVSRIQHHVPLAEPVVKEFLPDSAVVGYALERAKTQPIRDFLLYHIYPTPPPPPPPNTVPPLTASADHSTFLDGRPRRLSDLLLNILNVTIAQWETKTKPIISSGERPRRCIDLVVLGLAFQGLLQLDGYIPHAACINAAIRLLHLLKPLLISSDLSILSLDLVWRGLRCLVDVPKEGKEEEEEEDWPILVKPDVHSGIRQDLLPPTMYDTPPQEDGEEGTSSDPAPAPAPAPVLVPGENGDGAKQQSTQYPPTFPSTLLPMPMPMSTAPSSFSTFQVQSNPASLVHAIWRLPEKTQVSATLQGLFPVCLQVITRSPSSMSTGQPTQLLSSSSHHHDEEGHDEDEEDDDFAVTSGEATSAPLPEKVAELKASASLLRSAVAFRLQGVMMLVSGGGGGGQSKAYKDTQLINSFLQADGLRAVEIGWAICKAMQKGWLRLSVDAVELVVASLGNMLNSYGYARDERLLQLCLEFLKCSAPVWMGSNSGSGNDDLMDEAMRLVCYIATKITSGSITSWKVRLAMLRFIEQFLHYDSASTLWADGMVQEEAEEHDVSMEDEEHLFHYISQSLSDPDMRVRTRAATTAASALYRPSIHPSEHPIIYDQASNSQAGDPTFPEHYLSYVLWKLNCCIASAKQRQMVIFDLYETAIGGAEYSDHLRAGLDAVARRLGLSSITALYLPYAPTTTISHDTSRSSAITFVLSTTHRLFGLSNRSAFFTACLEHAGAYILYCGKIGLFISACDAAGGVSPEDLALQLSVAAAAMAMVLFLSNDKTNVVNMKECKAEALALLSTFPGMSGPADAEKLLHNYVNGVAAHLWELMDLESSVDEIVAWLDKTEKDTAAGIAFAQMMAHDNNAKTGKVKAINPAASFQNIYNVCRFLEKEYSSISLHALTFAAILRLTSLINNAFLVSEQRRYLRALAMLLSVHQRTLQRPLIWEAFLTETIALLLQPDICRTVLSMVMWAFDRLESLSSTPSKLVNIFCQLGEIRIELGGSAATGSHQSNQMGDAVEEWIVKMSPKWSNSQISQEAFERAVALWPDSLRSRLSVLATPLSLRDLDDLSQNKAVHNAGQLCKQFLRRADSDDRQDVVSVFVDSTFWSLKDKISSVLDKEGINAFQDLLYLCNGEVRAPSLNFYGQDTSAKALTATTGEPRKTEAMNALYLAICKTMVQLLHDRRPQVRSAAYRCLQRMKPILTGKDLKELPADISDVLPILVPIPVGTVSQSQAMKLDGVINNAAWSNKAEHFATWSLELSGLLCKVASQHDKFFLSFEPLLSTPLLPLHHFLGHFVHAALVCSRSMSSERSKAISEHFETVLRNPFASTETVQSIVNIVLQLRRYEHPFRSGMLGYNAWLSVNFVDLSKAAVKCGLYVSALLFLELANDQGGWLDLGEPRVREIMYDIYSNVEDPDGFYGVQNKDIRDSLRRRLEHEGLSWEALGWAGAVYNVNGSDSRSAIPVLHHLHDIGLSRLASVVATETRTSGSVPLDDPFFADLSWRTGDWNLPIGRESGATSSGLLYSALSAVHRSKSYESASKIVDKAVHAEMTRLGGLQKEMLTSIQSTVTNLLCLRELNRWLDPQLQQEIHEAIDRGTLRDLQDINDRFEFASAERIIATRLSVFDSVKQRESRDMIGDVLTPKMELVIKAESTCHIKLSRLALKSNNLQAAINSLTALQKLQADVGEIDEAQDVFCEVLWKQGEHTLAIQLLEDLLLREKKKKSKGQRIPALEGRLAHWASEARLKAAHEIFGMFSNVTKSIKRSTADVSEHAEIFYQFACFADKQYVSQSSSADVKELKEYSKLRASQVLRLSARQSRARESDQKNSAVREAELDEEKLKKFEMQQKQYLNAALQFYAEAVSMTDNFNNCITRLVTLWLENDKNEESNVIFSRAIRKVPSYKFIFLAPQLAARLHRPESPTIFNATLNGLMFRMSQDHPYHTLYHVIPLLWEHKQPQSTNSSMLGRKSAADDIMMRLTSSASNRLAAGAARSMKRFVAIAMEWTSFVEKDKRLEYKLPSDSPLRKTPRDIPVATSAPSIDVTCQYKDIATFDHFSEWYTRAGGLSRPKVMTCFDSNGQKYTQLFKKDDGFRQDAVMEQIFVLVNDLLNRNRQTRSRKLRYRTYGVLALPDATGVIEFVVGTKPLIKYLPPAHEKYHPKDITSHDFLKTMQEVQSVKNNDEKIIQTWMKLKKRFHPVMRHLFTEKYRDPMAWFSMKLTYARSLAVTSIVGWVLEIGDRHCSNILMDECTGELVHIDFGIAFGAGRILPIPELVPFRLTDDLVDALGVTGVNGAFRQCSQLVLQTLIDSSDVVLTILEVFKQDPLHTWMVDDKMKKAQDGHHKMYPERGQEKADRIMRETRENLSKELSVQYRVNQLIQEARDVNNLATIFRGWHSWL
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 15131
B YPD 30°C, concentration: 5x10^7 cells/ml 4125
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 5439
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 3383
E YPD 37°C, concentration: 5x10^7 cells/ml 4870
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 11464
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_05771 5' flanking region primer 1ACCCTCCATACATCCTTCC
L2CNAG_05771 5' flanking region primer 2TCACTGGCCGTCGTTTTACGGCTATCGTTTCGGTAAGG
R1CNAG_05771 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCAGTATGGATGGGGAGTAATAG
R2CNAG_05771 3' flanking region primer 2AACTCCCAAAGATGAGCC
SOCNAG_05771 diagnostic screening primer, pairing with B79TAGCAGCAAAAGTGAGCG
POCNAG_05771 Southern blot probe primerGAAATCGTCAAACTCGTTCC
STMNAT#225 STM primerCCATAGAACTAGCTAAAGCA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_05771T0
Mating Data
Growth phenotype for CNAG_05771T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_05771T0
Urease
Urease phenotype for CNAG_05771T0
Melanin
Melanin phenotype for CNAG_05771T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_05771T0
Oxidative Stress
Oxidative stress phenotype for CNAG_05771T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_05771T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_05771T0
ER Stress
ER stress phenotype for CNAG_05771T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_05771T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_05771T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_05771T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_05771T0