Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_05935T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationUMP-CMP kinase
Gene SymbolUnknown
Kinase SuperfamilyP-loop containing nucleoside triphosphate hydrolases
Kinase FamilyNucleotide and nucleoside kinases
KEGG Linkout KEGG::CNF00400 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction N/A
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K13800 KEGG 4.3e-95 317
Pfam Annotation
Pfam AccessionE-valueScore
PF00406 Pfam 4.6e-44 147.9
Amino Acid SequenceMTVIDTIKDKLHHHHHHDKHADADAEPAAAAAQTQTQTQTTPTPVSPETSKDASAAPDVEFSDKPVFDANKVTVIFVLGGPGAGKGTQCEKLVTEYGFKHLSAGDLLRAERSREGSKYGAMITEYITEGKIVPMEVTIKLLENAMTETLSSPPSASGWSNGFGRFLIDGFPRKMDQALKFDESVCKSSFVLFFSTSEEILLQRLLERGKTSGREDDNKESIVKRFRTFLETSMPVVDYYRERNKVVEIDSSPSIDEVYAVVKREIDARLPKRGPAYE
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 5753
B YPD 30°C, concentration: 5x10^7 cells/ml 10613
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 9247
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 13190
E YPD 37°C, concentration: 5x10^7 cells/ml 8405
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 3954
Gene Disruption Strategy
Primer NameDescriptionPrimer Sequence
L1CNAG_05935 5' flanking region primer 1GGTCAATCCAGATGCTATCAG
L2CNAG_05935 5' flanking region primer 2TCACTGGCCGTCGTTTTACTTTGGGTTTGGGTTTGGGCAGC
R1CNAG_05935 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCCCGTGTTGTTCTTTCGTAG
R2CNAG_05935 3' flanking region primer 2CAAGGGTGTTGGTATCTACG
SOCNAG_05935 diagnostic screening primer, pairing with B79CGGAAGATTACATCCTGGG
POCNAG_05935 Southern blot probe primerTTACTCATACGCAGGACCC
STMNAT#220 STM primerCAGATCTCGAACGATACCCA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG