Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_06161T1
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolVRK1
Kinase SuperfamilySAICAR synthase-like
Kinase FamilyPhosphatidylinositol phosphate kinase IIbeta, PIPK IIbeta
KEGG Linkout KEGG::CNM01630 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction N/A
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K00921 KEGG 1e-156 514.3
Pfam Annotation
Pfam AccessionE-valueScore
PF01504 Pfam 1.8e-48 133.4
Amino Acid SequenceMDSSRHNLHGLPPTYLAHLKALLHQWLTQQTTPAGDSKLWSEKRVAQIERAIWEGAVLTSAERGSRRESGILEQNWEGWIGGSTKRKSLWKEETRQEEEKIKEDEKTEAHVSRKSSMGDRDRGRLRLMVAQAGSTASTPSNASPRASSVASTERRQGTDSAPRDVNKGKTPEQSDQEKLQEWCRIVSTLDGYEPLSLAKADGWEDINTDRVPKSTHQHIPGSFPQSGLSIEYRHSLDNPSQSKGTFSTLGLPSSPPKHDPSNQSYDLLKPVFCLHFPISHSSRTSTKGLARKEGSQSTIRIRRDPSVSARSASSAGSSRWWGSGNKWGDILGSPFLQDDEEERVEVDFVQGRFALPSDLFGSPALERRESKKGRVTKRKRRRSIFSALEVVSSSLSTNSSLPSESTSDTTSEAESGQSDTDTDDEMSGKEIKRRIPSRRHRPLSLILSGAEDDIPRSAAEEGKIRLVGGTIVVKGLRGGERKALGEVLKALIWTIGMMKLELDLYTAFRLPHEPDPSTTFALDVSSSVPLIHPALSPQTSNPTPRPSGEVSRSSSLREKDRSRGQGSREGKKGFLSKLGKNTKGVWNGLLGRKGSARDKSHGQSDMGGEEYTSPILSPTLPSESSPSSPLFPSQPLSSHLPSPSLSSPSSFPRFKYSSYPKDPSPDSNSSFHTNPNINTDANSNANTNANPPSQHLQTLSTLSTLLPSSTPGLKYPMPPILLRIQEEERIRREKTQKETLAMSENSNGSGDTSGNTLRGRAMAYRPGGDVRSGLHVLTAGIDTLAGWTRLQLLCVLYCVGLPSPVSGSKSGSIANGGEQLGEDVKGEADEEKGGEGEICESPQPQFLPPTLTIGGYLQHLGEELGENLLCTRSGCEKEVGQHVRWYVHAGVKVGLRIENVKVKEDGGRDDDGVREDEDEGAVAGKAEDGQDVEGWVKCIVCREESEPRQLNEGALAYPWSKLLELLIYTPHLQPPICDHHTTPHAYITLFRTSSYTASLTSSPVKLYDVRLPKLQIGPNIPKRKAGGGRQGMGLMMDDEKEGENALKREVTRWCEALDDRINILREKIEREYEAAKELSEGPEKSGPGLQEKFDTLLSLSTIFTAFKDNSLESISSTPQDLFNDLRISFSAFIRSSLSEIEAWEKKYLPENAEEGLETMKEMIPEYAKEGGRVHALPGSGVLVKEDEPASVVAYTLSSLAYLIELTNTSKPADDSNPATAITTPRDPTDSSLPIAPTTGAGTPTSASTPSGSGWSTKIEHRDSPRDLLSLRSIVKKKSDASVSFDKAKFTPSALGLSTLSGGAPSLELNLEKVEGVSEGVEGRDRMEEIAKAVGKATGQEMTLGTTTAHAVPSSLSRRASMTESDTSSSSGVRLSSPATKSSQGPPSSFRPIRSASSTPISRSPMSPLPTATRSPASGATTTSTLSPNTAISPGARDSSWDSVTSSFVNSVNNLWKLGSEMGETFSSMRSRPRDQSLKSLMGPLSSMDNSLSTLSPRPHLHFTYTFADKLRLSCTVYFATAFDTLRRRCAIDKVLVQSLERTEVWDAQGGKSKAGFWMTKDKRFIVKELLSKWTVSDMHALLEISPAYFHHMAGTHNRATALAKIVGFYTVTIKDSQCGQKRQLDLLVMENLFHNQNILKTFDLKGIEGRRVAKTKEGDKEVKSTTTQFDADWLEGMQKGLVLLQPHAKRILLDAISLDTRFLSSQSIMDYSLLLGVDTRSSLLPSPNISEDKLDQERENGLLIVGIVDAIGSFNLFKTIESRGKMVMNRGGDVTVIPPDQYRERFENAIRHYFVACPDKWSKVSRKVGGFNGCEVSSVL
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 11179
B YPD 30°C, concentration: 5x10^7 cells/ml 3046
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 3272
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 2808
E YPD 37°C, concentration: 5x10^7 cells/ml 2218
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 8701
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_06161 5' flanking region primer 1TATCGGCAGCGACTCTACTC
L2CNAG_06161 5' flanking region primer 2TCACTGGCCGTCGTTTTACCGCAACCATCAACCTAAGC
R1CNAG_06161 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGATAGACGCCAAACGCATC
R2CNAG_06161 3' flanking region primer 2CCAACCCAACTACTACATACTGC
SOCNAG_06161 diagnostic screening primer, pairing with B79GAAGAACTGGAAGCATTGG
POCNAG_06161 Southern blot probe primerCGAGAAGAGTGAGAAATGGG
STMNAT#123 STM primerCTATCGACCAACCAACACAG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_06161T1
Mating Data
Growth phenotype for CNAG_06161T1
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_06161T1
Urease
Urease phenotype for CNAG_06161T1
Melanin
Melanin phenotype for CNAG_06161T1
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_06161T1
Oxidative Stress
Oxidative stress phenotype for CNAG_06161T1
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_06161T1
Cell Wall Stress
Cell wall stress phenotype for CNAG_06161T1
ER Stress
ER stress phenotype for CNAG_06161T1
Genotoxic Stress
Genotoxic stress phenotype for CNAG_06161T1
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_06161T1
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_06161T1
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_06161T1