Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_06174T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationPEK/GCN2 protein kinase
Gene SymbolUnknown
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNM01750 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
STE45.12.9e-16
Microbial Kinome Result
PredictionScoreE-value
ePK248.04.6e-74
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K16196 KEGG 0 1018.7
Pfam Annotation
Pfam AccessionE-valueScore
PF05773 Pfam 3.1e-21 73
PF12745 Pfam 1.4e-25 85
Amino Acid SequenceMPEANKALQEEELESLRAIYPDEWHDIPPTKTAWGTEVDAGWWEVKICAMEDERVSVILKGKMIQAYPHQVPPLLLREPEYLTANHVQQLHRIIQDKAKSKVGEVMIFELIDTVRDFISENHAPLPSPGDVNLMEEKARREEAQRAAEEASRATEAERKKKELDKNNRYLHDQIQLNTIKKKETANNAKYHEEERRRQESLATLDVGDLDKRELELDEAIALDGWEGTWKSWILFGGKRETLWTTYTAEPDRKHNSSQRSDMASFVTASSPNVTVHIVDFANPYYSTALGIKRIEACTVEALRAQGTMSEHVVKVYAVKREKSPKGWERLILVTEGLSDSMKLRSWMPKEGFGEDLSKEYISQALIGLSQLHQRNSCQKQLDLDFTLISTTPNGTKVVKLAGTGYARRIVDLHRSNPFIKNVIQTVPEEWLSPDERDSPHSYTRPRDMWHVGLLLIQMLFGPETLRTYHSFSTLLQHAPRLSDSMTELLTGLLHPNPKKRLTADESLAKLRSFDDVTRRAVRISSAPGHARHQSIPQSPQVLLGVSPVNRSLLNFLPNTPQPLTPRLSRYRADFEEVEFLGKGGFGEVVKARNKLDGRFYAIKKVKLRPEDNEQKVYREVNNLSRVNHQHIVRYYGCWLEDANPPDLTPTLEDSTHAASTTGTGATCSTEEDIFAVNFDDFSLSRRDQSRSASFPRIRFTNSNGDDDDDDSDEGDSSEEDDTESDTESDSSAATADPSEPRGRSHPQSSLFQQGPEARSQRQSIPGKPSASTSVMTSSTSDGMVRRILYIQMEFVEKQTLREAITAGLTDDEVWRLLRQVLSALAHMASLGIVHRDLKPSNILLDGDGNVKIADFGLSTSEMTAIEIASGPIASTIDNIDQTSNIGTSLYIAPEVAISRSYNEKADMYSLGIIFFEMCYPFKTAMERVHILTAVRQPSISFPPGWGPNHKSNEKEIIRRLLAHDPAARFKATELLRSPLLPTPEKRKEDYDAVIFELTDPKSSHYGSLLDTLFDRTSHNIVDVNHRLVDYTYDNDSDDQLQVWLTVVIQRLVDLLQRHGAVETYLPLLIPETTLLDAFPNLDPVRLIEKSGQVVQLPSSDVLAMGRSATRRQIERIKRYHVGRKYTEHQLGGQPVVSGELSFDIVSPIRSAAAEAECLEVVDKVISEFRGMRGTSSVEYEFHVSHETVLDAILGIIPDRPDKPRLRVLEQFRNLSASNSANASAQSRNLLSNVTGLSRMLMDELEQCCIADDFEVVRKRLESIFTSASAKRKLATALDDMTNVINSARACGVARKILFRPTLAKHSEFFRGGFMFECVRKGKQKEVVAYGGRYDSLLEHFKQPAMHSQSRRVFGVGMSIAVDQLARTVSRYESSLSRRLMEKPNEDERSFGYWSPARCDVYVASFPQVDLAHRLQIIGELWRAGIRADFQYDDGRSLEEVAQECQDQNILYLVIPKASRSTIKIRSILRRSEVEVPRNDLCNHLRIVISDQRRIDASYATAEGSIPSAQAAALSVEPKQAEVEIKLILPPEPMSAKGTKGRPVRKHRHGTKSVYYDKASDFATQTQSTLPVLGIDLPPLLICQLTLDTAWITDDEAWRSLLNKEGVHPGDKRYMESIREGVQQMRAGGAGNGGGGGGGGGSGGGGGGGVGSIGRENGWMWLFSVRDNKGFLLQVGHGK
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 7639
B YPD 30°C, concentration: 5x10^7 cells/ml 5637
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 5381
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 5626
E YPD 37°C, concentration: 5x10^7 cells/ml 5955
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 6326
Gene Disruption Strategy
Primer NameDescriptionPrimer Sequence
L1CNAG_06174 5' flanking region primer 1GCTCACATCGTAACGGTTG
L2CNAG_06174 5' flanking region primer 2TCACTGGCCGTCGTTTTACAATGAGCCGAGAACTTACG
R1CNAG_06174 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTTGGAGGGCTTTGTTAGC
R2CNAG_06174 3' flanking region primer 2GCTCAACAACAACAGCAAGAG
SOCNAG_06174 diagnostic screening primer, pairing with B79TCCGATGCTCACGAATAC
POCNAG_06174 Southern blot probe primerGTCTCGCACTGTATCAATAAGC
STMNAT#119 STM primerCTCCCCACATAAAGAGAGCTAAAC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG