Kinase Browser
Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.
Detailed Locus Information for CNAG_00826T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
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| Gene Annotation | dihydroxyacetone kinase | |||||||||
| Gene Symbol | DAK101 | |||||||||
| Kinase Superfamily | DAK1/DegV-like | |||||||||
| Kinase Family | DAK1 | |||||||||
| KEGG Linkout | KEGG::CNA08050 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MTDRHIFPDHKTLVFRSIRGLVASHPYLSLIPSQKVVYRADHDPSKVSLICGGGSGHEPGTVGFVGAGLLTASVAGDVFASPSARQVMEAIKRVHSDKGTILIITNYTGDNLHFGLAKLMAQSAGLKNVELVVVGDDVSVPKSRGKYVGRRCLAGITLVCKILGAASEVDVEFQDLVTLGRSLSANTASVAMALDHCHVPGRSGEADWHLPEGKVEIGLGLHNETGVFSIAQPPPDVLINKLLDLLLKQDDPERSFVKFKDGDELVLLVNNMGGMSVLEMGSVVDEVLIQLESRGIIPTRILNGPFMGSMNMPGISLSLLNLTNVAEECSFVDTSKLIGFLDAPHNSVAWPATSQVYPLPEKLANRKREDKFVDAEEEKKEEITGGPQLFGDKQFILKAMKQAAEDVLASEPKLTKWDTIVGDGDCGETCALGAQATLKALEQGLGSDGELVHFFRVLTEVIDGSMGGTLGAIFSIFLAGLTTALIDAASSSNGTPKLTPAFFGQVTSSALETLKARTAARVGHRTVMDALIPFGETLAESGDLKKAVEACRKGGESTVDLQAKLGRATYVGQLEGEMPPDPGAMAFVTVAEGILKAYSS | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 6037 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 1121 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 1056 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 1140 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 937 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 9814 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_00826 5' flanking region primer 1 | AGTTTGAATGAAGGGGCG |
| L2 | CNAG_00826 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACGGAAGATGTGTCGGTCTGTC |
| R1 | CNAG_00826 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCGGAAGGTATTCTCAAGGC |
| R2 | CNAG_00826 3' flanking region primer 2 | GCTGTTCAGTTTCCTCTCTATG |
| SO | CNAG_00826 diagnostic screening primer, pairing with B79 | ACAGCGATGTGGGGATAAG |
| PO | CNAG_00826 Southern blot probe primer | CATACTTTCCTCGGGATTTC |
| STM | NAT#282 STM primer | TCTCTATAGCAAAACCAATC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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