Kinase Browser
Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.
Detailed Locus Information for CNAG_06454T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | Atypical/RIO/RIO1 protein kinase | ||||||||||||||||||
| Gene Symbol | Unknown | ||||||||||||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | ||||||||||||||||||
| Kinase Family | RIO1-like kinases | ||||||||||||||||||
| KEGG Linkout | KEGG::CNN01780 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MSRRPDLGYIDQASEDPVNTVSTPVEDLGQTPHEGVEAEAPEEDSGSSIEEESEGEPFEAVDGDDFDFSALNAGGSGEEEEEEDDGDWDVDDEDWELANGDFTKQYNRVRQQHAATSGSAPLPARNLSQQTKSKLSKSNPTAGSGVVTNPKAAQDKHDKDKSDRATQEQVLDGRTRLVLAGLVNRGVIGMIERCISTGKEANVYYSSPNRAVKIYRTSILVFRARQNYIVGEQRFRGEYTSSRNPRKMIRVWAEKELRNLRRLVQGGVRAPVVHECKENVLVMDFLGKGEVASPRLKDAEIPEDRLPDLYAEMIIATRRMYQHCHLVHADLSEYNILLHDNHLYIIDVSQSVEHDHPRAFDFLRSDISNIEEFFSRRGVATLGIRKSWEFIVTENIGLSPTGSASSSLEMEKGDQGERRLVDVLEKWLKEPSDKTDDAVFMESYIPRTLAEVYDPERDVDVLKSGGGDELIYAGVTGLKLAHESANAKEKEKEKEKEKQSASAGANKGEGVDVDESVEKGKKGKGKKSKDEEGKNEKSPKSVRFEDEVDEEQDEDDDAQKAEEEDEEEGMDKKSRGFRHEDRESKKERKKAVKEEQREKRKTKMPKAEKQKLIKKSASRH | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1749 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 2573 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 2560 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 3398 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3322 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1639 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_06454 5' flanking region primer 1 | AACAAAACCGCTGGCAACACCC |
| L2 | CNAG_06454 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTCCAGAGTCTTCTTCAGGCG |
| R1 | CNAG_06454 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGACCAAGATGCCAAAAGC |
| R2 | CNAG_06454 3' flanking region primer 2 | AATGGTTGACAAGCGTGCC |
| SO | CNAG_06454 diagnostic screening primer, pairing with B79 | ACCCCTTACTGGCGAAAAC |
| PO | CNAG_06454 Southern blot probe primer | GGCAAAACTTACACCTCGC |
| STM | NAT#232 STM primer | CTTTAAAGGTGGTTTGTG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |