Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_06642T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationAtypical/PIKK/FRAP protein kinase
Gene SymbolUnknown
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyPhoshoinositide 3-kinase (PI3K), catalytic domain
KEGG Linkout KEGG::CNF03740 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
PIKK359.13.1e-107
Microbial Kinome Result
PredictionScoreE-value
PI3K_Sugen189.22.3e-56
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K07203 KEGG 0 3020.1
Pfam Annotation
Pfam AccessionE-valueScore
PF00454 Pfam 4.1e-72 240.8
PF02259 Pfam 1.2e-116 386.7
PF08771 Pfam 1.6e-39 131
PF11865 Pfam 4.2e-55 171.5
Amino Acid SequenceMSSQSDVLDNIFQRLSARSEDVRAQAGQDLAEHVVAYTQEYPGHDASKGVWAQVFHKTFEFTRSNNQLERLGAIIAISQLLQLTKDDTPDRAQQKVLRLYEYLRPLTTCGDSTVMLPASLVVEDMVRNSPTLHTDTFLGKEVGQALVMIDDSRQEVGRFSGALLLYAFARAAPGVFHQYISKVLEKIWIPLRDSRSVVRERASMLLSTCLDTLKTRGDRPSTDTYRKIFEEARLGLLKASSTESILGSLLAFNSMLQNQQLSMAEYYRSICELTFKYRDSKEVSIRKAVIALIPSMATYDSDDFEAHYLHRGMAYLLQALNRPADRDISYVALGHMAVHLGSKMKPFIDDIMRIIRDHLRMRGKKNAPYEAPIFQCLAMLATSVGPMLTRQMHEILDLMFPWGLSEPLCTALQATASHIPPLLRTIQDRLLEMLSQTLTGQSYRPLGAPAPRGGAQMDLNLLQSTTNAQSTDTLKLALRLLARFDFVGHTLSEFVRDAALPYLEHDSVEVRREAVLATTTLFMTDPICQQTSSNSVEIVNDVLSKLLTVAITDPNAGIRRTVLDHLEDKFDRHLAQAADIRCLFIALNDEVFGNRERTISIIGRLAHHNPAYVMPHLRKSLINIVTELEYSTNARQKEESAKLLCLMIGAAAGLVKSYAPTILSVLLRTASSPESSIGVQAECLKCIGELARVAGEELVPSVRAILDLVIEMLNDQSSPAKRDTALKTLGQIASNTGEVIKPYTDYPQLMGVLFRFLRMEANSSVRQETIKTIGMLGALDPFKHKTLLGDVDDPIDEGTTSRVNDIVLLNQHNSSVNDEFFQTVVIHSLVNVLHDSTYKDHYQAVEAIMMIFRTQRLRCVNFLPQIVPAFLNVIRIAHSSRTELYLKQLAQFITIVKLHIRNYLNDVFDLIHEFWNPNSTLQITIISLVEAIAKAVEGEFKAYLPKLLQQILRSFDGDLSAKHLPELKLNTLLQILKAFYVFGESIEDYLHLVLPVIVRSFENPAAPDSLRIAALRTTGQLCRKVNFSDHASQIIHPLVRTLGNSSEELRQTAMETLCVLVLQFGPDYAIFIPMVNKALVENKISHPGYEALITKLLNRERLPPDLGPVERYASDSAAEASAPEPVALKVNQQALKLAWDCSHLLNTSSRTEWISWIIGLGHEMMRESPSQAIRAARSLALSSVAFTKELFNVAFYSCWQELFESYQEDLWHNLDRAIKKDDVPGDVVNMILGATQFLEHDEKEVAIESRVLGSVAANYQALAVALHYKEQEFFLDPSKEVIEDLIDVNQKLQQSDAAWGTLEWAQTEMGMTTEVEWYEKLGRWEEALQVWNERDADASTTFSEWEITEGKVTCLHAMGEWEQLSDFVQARWANRTAEEKKLLSPLAAAASWSLKQWDLMDDYISAMKGDGADRAFFKAILAVHRNQIPAALKQISKARERLDPELTTLTGDSYGRAYDTVVRIQMLAELEEIIAYKDHADEPARQEMQRQTWKKRLAGCQRDVEVWQRILQVRSLVLKPNEDMDTWIEFADLCRTSDRLNLAEKTLTSLVGFQYPSMEDTRGRAPPPIIFAYLRMAWAKNLQIDSREERYETLQHLRDFTDQLTDDVGIGARGPNGRLMLPDQKLYGSYTKLLAQCHVELGQWQATLRESQGSADPSGILHDYCLATELDPEWYQAWHTWALANFEVITQLEVSQQGLSPVHFTTYIIPAVEGFLKSISLSPGNSLQDTLRLLTLWFTYGYSSGVTAAVSQGLPTVNIDVWLEVIPQIIARIQTPRQSIQQLIVQLLHDIGKAHPQALIYPLTVASKSTVAARRTVAQNITHKMREHSPKIVDQAELVSTELIRAAILWHEMWYDGLEEASKHYFGDHDIPGMLGVLEPLHEIVENGPQTLRETSFIQSFGHDLRIAREHLKRYRITQDGTEIQQAWDVYYSVFQRLGKQLKLLNVIELQYVSPKLMAVRDLDIAVPGTYQSGKPIIGIKNVIPTFKVIASKQKPRQCSMRGMDGKEYAYCLKGHEDLRQDERVMQLFGLVNTLLNNDHESAKRHLSIQRFSVTPLSPSAGLLGWVTHSDTIHVLIKQYRDQRKILVDIEHKLMQQMSDESYDSLPLLHKVEIFQYALDNTTGQDLYRILWLKSRNSDIWLERRTTYTRSLGLNSMVGYILGLGDRHPSNLLLDQITGKMVHIDFGDCFEVAQQRDKYPEKVPFRLTRMLIHAMEVCGITGNFSRSCEVSMEVLRDNRESLMAVLEAFVYDPLIAWRLTATDKRPGGVGEVKDLDDPAVYGKQRKNKANETEILNDVENTEVKNDKGLQVIERVRRKLTGRDFKPDVVLDVKSQVEKLVVEATKTENLCVAFLGWCSFW
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 27566
B YPD 30°C, concentration: 5x10^7 cells/ml 18025
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 20143
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 13160
E YPD 37°C, concentration: 5x10^7 cells/ml 13013
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 18823
Gene Disruption Strategy
Primer NameDescriptionPrimer Sequence
L1CNAG_06642 5' flanking region primer 1CCTTTTCCTTTTACCTGGC
L2CNAG_06642 5' flanking region primer 2TCACTGGCCGTCGTTTTACCGCTGAAAGATGTTGTCG
R1CNAG_06642 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGGATTGACTGGACGAAAC
R2CNAG_06642 3' flanking region primer 2CTGGTATGCGTAAAGACTTGAC
SOCNAG_06642 diagnostic screening primer, pairing with B79CCTGCTGAACGGATGATAG
POCNAG_06642 Southern blot probe primerGAAGGTTAGTTCGCAAATGG
STMNAT#43 STM primerCCAGCTACCAATCACGCTAC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG