Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_00130T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationCAMK/CAMK1/CAMK1-RCK protein kinase
Gene SymbolHRK1
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNA01180 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
CAMK242.93e-72
Microbial Kinome Result
PredictionScoreE-value
ePK305.62.1e-91
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K23785 KEGG 2.5e-275 915.2
Amino Acid SequenceMLSSFKQILRHGKHAQQASPSPQPDTKSGRSKTTQTQPQQPMPAVQQQAQQPSQPQQQQQHRRNDEYSSRLTTSTAQQNKVPAASPNYREEAEKIVADERAQSEKMPVYAGLEEFKLIEKMGDGAFSNVYKAVDRRSGQKVAVKVVRKYELNQSQHGNKHLNANFKKRGPRVTERANILKEVQIMRGIDHPSIVKLLKFFESDEHYFLVLELMEGGELFHQIVKLTYFSEALSRHVILQVAEGIRYLHEERGVVHRDIKPENLLFERIPIIPSRNPVHRPYDEEKEDEGEFQPGIGGGEIGRVKIADFGLSKIVWDEQTMTPCGTVGYTAPEIVKDERYSKSVDMWALGCVLYTLLCGFPPFYDESINVLTEKVARGYYTFLSPWWDDISHSAKDLISHLLCVDPAQRYTIDEFLAHPWIRDAAPPPPPPTSRPMPSESPMYSPLLASIRAGNREARSPGVGALKEAFDVTYAVHRMEEEGARRRAYNGPGGAGQRGFLHGLNEEAEEEDEQAQVEEARRKHGEAVARQIQEHRGRAAANAAAGIKEPPVTNYVGRGGANRREAEAVIYDGRAGQRDRGKGGKSAGGFDLDLNNATLLGRRGKKVAPSPLAQQVQAGQ
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 140771
B YPD 30°C, concentration: 5x10^7 cells/ml 33802
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 37898
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 17926
E YPD 37°C, concentration: 5x10^7 cells/ml 16404
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 51556
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_00130 5' flanking region primer 1TTCCAGTCAACCGAGTAGC
L2CNAG_00130 5' flanking region primer 2CTGGCCGTCGTTTTACCTGTATTCATCATTGCGGC
R1CNAG_00130 3' flanking region primer 1GTCATAGCTGTTTCCTGCGTCAAATCCAAGAACATCGTG
R2CNAG_00130 3' flanking region primer 2GCCTTCATCGTCGTTAGAC
SOCNAG_00130 diagnostic screening primer, pairing with B79AAGACGACCACATCTCAGAG
PO1CNAG_00130 Southern blot probe primer 1AGGACTCTGCTCCATCAAG
PO2CNAG_00130 Southern blot probe primer 2GAAAGAGCCTCAGAAAAGTAGG
STMNAT#58 STM primerCGCAAAATCACTAGCCCTATAGCG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_00130T0
Mating Data
Growth phenotype for CNAG_00130T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_00130T0
Urease
Urease phenotype for CNAG_00130T0
Melanin
Melanin phenotype for CNAG_00130T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_00130T0
Oxidative Stress
Oxidative stress phenotype for CNAG_00130T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_00130T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_00130T0
ER Stress
ER stress phenotype for CNAG_00130T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_00130T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_00130T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_00130T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_00130T0