Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_01730T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationSTE/STE7/MEK1 protein kinase
Gene SymbolSTE7
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNC02350 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
STE253.22.2e-75
Microbial Kinome Result
PredictionScoreE-value
ePK235.42.9e-70
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K11226 KEGG 1.1e-152 508.2
Amino Acid SequenceMHPQNPSTPSSNSSSTFSLPKSSISRKKPPGLDISKSILRPTRTAPVLPSNGSLPSNFMSEADKLRDDIANLQLSSRSTGSNHSLEAFDSPRAFQSDSSVASGPAKKESRDGKERGDKNKRKKKHKDKDGEDLVRDEDLEILHDLGAGNGGTVTKVWNKKRKCVMARKLILVDAKPSIRKQILRELQIMNDCDSPYIVAYYGCFPVDVHVGIVMEFMDAGSLDYIYRHHGAIDIDIVGKVAEAVLEGLVYLYDKHRIIHRDIKPSNVLANTRGEIKICDFGVSGELINSIANTFVGTSTYMSPERIQGAPYTIKSDIWSLGISLIELAVGRFPFSDSPDSEELSPSVSDFDPDPTLPRSVRRPKIESNSGRNGVSQSGGPHAMSILDLLQHIVNEPAPRLASRRRTFPQEAVAFVEGCLIKDPDQRRSPKELLSSPWMTNLKPTKEDLQAWAQSMTGPREE
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 724
B YPD 30°C, concentration: 5x10^7 cells/ml 1132
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 1279
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 1217
E YPD 37°C, concentration: 5x10^7 cells/ml 1001
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 1117
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_01730 5' flanking region primer 1TTGTAAGGCTCTCATTCGC
L2CNAG_01730 5' flanking region primer 2CTGGCCGTCGTTTTACTGAAGGCAAAACTGGTGC
R1CNAG_01730 3' flanking region primer 1GTCATAGCTGTTTCCTGCCTTACCGTGCTTTTCTGC
R2CNAG_01730 3' flanking region primer 2TTACTTCCGCCCAACGACAC
SOCNAG_01730 diagnostic screening primer, pairing with B79TCCTCGCTCACAAAATGGGC
PO1CNAG_01730 Southern blot probe primer 1CCAATAGACATCAAGCCGTC
PO2CNAG_01730 Southern blot probe primer 2AAACAGAGAAGAGAAGGGACC
STMNAT#225 STM primerCCATAGAACTAGCTAAAGCA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_01730T0
Mating Data
Growth phenotype for CNAG_01730T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_01730T0
Urease
Urease phenotype for CNAG_01730T0
Melanin
Melanin phenotype for CNAG_01730T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_01730T0
Oxidative Stress
Oxidative stress phenotype for CNAG_01730T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_01730T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_01730T0
ER Stress
ER stress phenotype for CNAG_01730T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_01730T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_01730T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_01730T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_01730T0