Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_01850T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationAtypical/HisK protein kinase
Gene SymbolTCO1
Kinase SuperfamilyHomodimeric domain of signal transducing histidine kinase
Kinase FamilyHomodimeric domain of signal transducing histidine kinase
KEGG Linkout KEGG::CNC03340 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction N/A
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K19691 KEGG 0 965.6
K19691 KEGG 0 491.8
Pfam Annotation
Pfam AccessionE-valueScore
PF00072 Pfam 3.5e-23 78.6
PF00512 Pfam 3.2e-18 61
PF02518 Pfam 9.4e-29 97.1
Amino Acid SequenceMSLPDAYPPVIATEGSTAFQSHILSLLSVISQHPSPDYPFPTSGDSTLQDNASVAAKKSATLSTFPGKKTPAEDAIERAIIALGDRVWTSERTQAHLVKDTKRDVPAQPGSDLLTPDWTPPVPTLSDSSPDPVCPTCARPVSSTESSSFTPQQIASFYHPFTAALSTPPPPLPEQMFFPQSVLAAYVSATSDSESAGDSGMSAEKELELLKAQVQDIARVCKAVATGDLTQKIIVPVKGQTMTELKNIINAMVDRLQTFAVEVERVSLEVGTEGKLGGQAVVPNVEGTWKVLTAVVNKLAANLTNQVRSIAKVTKAVAKGDLSETIDVEASGEIAELKTTVNGMVMSLRTLADEVSRVSLEVGSQGKLGGQANVPDVEGVWKDLTVNVNRMCESLTTQVRSIGSVTTAVARGDLSKMIEIEAEGEMAVLKNTVNSMVRQLTVFAKEVTRVALEVGTHGQLGGQAVVPGVEGVWDDLTTNVNKMARNLTDQVREIAMVTKAVARGDLSKTVNADVQGEILDLKCTVNEMVAQLTVFAAEVTRVSLEVGTEGKLGGQAVVPNVEGTWMVLTDNVNLMALNLTTQVRSVAEVTTAVAAGDLSKKINVAAFGEILELKNTVNNMVDSLRSFSSEVTRVAREVGTDGRLGGQAKVPGVAGTWKDLTDCVNIMAANLTEQVRTIAHATTAVARGDLTQKVVGVKVSGEILDLVNTINNMIDQLAIFAAEVTRVAREVGTEGKLGVQAEVENIEGTWQEITSNVNTMASNLTSQVRAFAQISAAATDGDFTRFITVEASGEMDSLKTKINQMVYNLRESIEKNTKARQEAEMANRSKSEFLANMSHEIRTPMNGIIGMTVLTLESELTRQQRENLMIVSSLAGSLLTIIDDILDISKIEAGRMTMEQIPFSLRVAVFSVLKTLCVKAAQNKLDLIFDVDPTMPDQLIGDPLRLRQVITNLIGNAVKFTTKGQVALSCRVKRYVAVADAVELEFCVADTGIGIKQDKLDVIFDTFAQADGSTTRKYGGTGLGLTISKRLVNLMSGDLWVESEYGQGSRFYFTTVAEMTSVPRDQIIERLSPWSGRSVLFIDTLGDQTGIVEMLQELGLKPIVIHSVNDVYLLQDQGLTVFDTMIVDSLKAAGELRGIEYLRYIPIVLLAPSNKPSGPSNPSFISLPESRRRLLALPSASEQLLSPIPVKDCLEMGINTYYTTPLSLQELSNAIVPALESHQIQPGDTVRDTILSVLLAEDNLVNQKLAVKLMEVAGHKIEVADNGEIALEKYKRRQLARTPFDVILMDVSMPVMGGMEATGLIREFEANEGVPRTPIIALTAHAMIGDKERCLAAGMDEYVTKPLRRGDLLASIAKVLAPKQSASGLEMPAVSIPANIYGR
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 9286
B YPD 30°C, concentration: 5x10^7 cells/ml 8121
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 8500
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 7584
E YPD 37°C, concentration: 5x10^7 cells/ml 7545
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 8853
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_01850 5' flanking region primer 1GTTTCTGCTTCCACCTCAC
L2CNAG_01850 5' flanking region primer 2CTGGCCGTCGTTTTACTTTACACACACGGGCGATGTCCTG
R1CNAG_01850 3' flanking region primer 1GTCATAGCTGTTTCCTGACTGAGCAAATCGGCGTAGG
R2CNAG_01850 3' flanking region primer 2AAGTGAGGGGCATTACAGG
SOCNAG_01850 diagnostic screening primer, pairing with B79CGACACAATACTCTAACTGCG
PO1CNAG_01850 Southern blot probe primer 1CTTTCGTCTTTGCCACAC
PO2CNAG_01850 Southern blot probe primer 2AATCACCCTTTGCTACGG
STMNAT#102 STM primerCCATAGCGATATCTACCCCAATCT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_01850T0
Mating Data
Growth phenotype for CNAG_01850T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_01850T0
Urease
Urease phenotype for CNAG_01850T0
Melanin
Melanin phenotype for CNAG_01850T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_01850T0
Oxidative Stress
Oxidative stress phenotype for CNAG_01850T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_01850T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_01850T0
ER Stress
ER stress phenotype for CNAG_01850T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_01850T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_01850T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_01850T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_01850T0