Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_01907T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationPLK/PLK1 protein kinase
Gene SymbolUnknown
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNK02500 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
CAMK232.92.9e-69
Microbial Kinome Result
PredictionScoreE-value
ePK316.41.2e-94
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K06660 KEGG 4.7e-236 783.1
Pfam Annotation
Pfam AccessionE-valueScore
PF00069 Pfam 2.6e-75 251.5
Amino Acid SequenceMAEQPAQAPAAAPVKKEKIVPPSPPLKIRDRDRGYEYMRVGFLGEGGFARVYEVQDQRASRRAVKVVSKASIKTKKNKTKLWAEIKLHQMLAHPNIVRFDDCFEDEENVYMILELCHHGSLMDLLRRRKRYTEPEARYYLVQLIAACQYMHQNNVIHRDLKLGNLFLDENMDIKVGDFGLAALIEKPGDRKKTICGTPNYIAPEVLFDTANGHSFEVDVWSVGVILYTLLIGKPPFQTKDVKAIYKRIRENRYEFPPEKEISSSAQELITLILNTNPDKRPNLDTILSHRWFLEGPFPAYIPASANDFAPDYRHISSSQSRRNFAALCHKSKIGIAQSINVEPARSRSALGPSIMQQERDFKNAVQPDSPISALLNSARQPLIQASASAIKEPSLLRKLSVAGAASTLSPVRKGAIGKENHELGRRPTAMERVGEESEEQRDAEEVKPMYNGITRESEVAAQKARIVSQMAAERQLYAERMEKASESAASGKHPVYQESRSRVAAAPRGTLPLAATTGQPTVSTFSTASSDTVSKSESRYAAYESSRPEKTRDFKTSLFEIFGQNLSTALAMAQTDEGFTSPNIEAKPQPPRVFVVSWLDYCTKYGMGFAMTDGTVSVHFNDSTSLVLAPGKRYFDDIRPAGADDLSQNIRRNYSIERYPSDLKNKVYLLKHFENYMLDKLFGDQPYTFEDKELRTEMVFVVRYLRMKHVILFRLSNDVLQFNFYDHTKLILSREGLVVTVIDRHSVMRTWSLEELLRPLDDDLSPKDKKRIEGIVHKVQYAKDVLAKIKSHTSSSKAAAASSSVPVSANTRSAAAVEREMGRPIR
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 224
B YPD 30°C, concentration: 5x10^7 cells/ml 3408
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 3509
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 5789
E YPD 37°C, concentration: 5x10^7 cells/ml 6087
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 506
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_01907 5' flanking region primer 1GCATTCTCTCAACTCGCTC
L2CNAG_01907 5' flanking region primer 2TCACTGGCCGTCGTTTTACTCGTAGCCTCTGTCTCTATCCC
R1CNAG_01907 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGAGTTTCAGCCAATACCAGG
R2CNAG_01907 3' flanking region primer 2TGAACCCCTTTGACCCATCC
SOCNAG_01907 diagnostic screening primer, pairing with B79CCTCTTCTGTATGCTGCGAG
POCNAG_01907 Southern blot probe primerTCTGGAATGGAGGCTTTC
STMNAT#282 STM primerTCTCTATAGCAAAACCAATC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG