Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_02202T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationadenylylsulfate kinase
Gene SymbolUnknown
Kinase SuperfamilyP-loop containing nucleoside triphosphate hydrolases
Kinase FamilyAdenosine-5'phosphosulfate kinase (APS kinase)
KEGG Linkout KEGG::CNE03380 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction N/A
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K00860 KEGG 4.5e-92 307.4
Pfam Annotation
Pfam AccessionE-valueScore
PF01583 Pfam 1.3e-68 228.4
Amino Acid SequenceMATNITFHPGAVTQDERDTLLGQKGCTVWLTGLSASGKSTIATALEQHLLHKKLHAYRLDGDNIRFGLNKDLGFDQASRVENIRRIGEVSLLFALSSTISVTAFISPYISDRQLARELHEKHSSAIPFIEVFIDAPLSVVEQRDPKGLYKKARAGEIKDFTGISAPYEAPANPEIHIRTDEVDVAGAVEIITKYLADNGLIPA
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 2153
B YPD 30°C, concentration: 5x10^7 cells/ml 3715
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 3092
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 5496
E YPD 37°C, concentration: 5x10^7 cells/ml 5069
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 1658
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_02202 5' flanking region primer 1AACAACCGAAACCAGCGAC
L2CNAG_02202 5' flanking region primer 2TCACTGGCCGTCGTTTTACGGAAGGTGATGTTTGTGGC
R1CNAG_02202 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCGCCGACAATGGTCTTATC
R2CNAG_02202 3' flanking region primer 2TCCTGGTCATCGTGCTAACC
SOCNAG_02202 diagnostic screening primer, pairing with B79CTTATGCCACTCCTAACCG
POCNAG_02202 Southern blot probe primerGCCGAGATACCTGTAAAGTCC
STMNAT#6 STM primerATAGCTACCACACGATAGCT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG