Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_02389T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationAGC protein kinase
Gene SymbolYPK101
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNE01630 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
AGC214.41.1e-63
Microbial Kinome Result
PredictionScoreE-value
ePK219.32e-65
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K13303 KEGG 3e-98 328.1
Amino Acid SequenceMATAVYHTKRPTLFASTFHSAADHSSSHNYADPYHPPTLQQSASVSKMTLIGVASVDVQSETAASLPEEVCPIEKASDDIKEPSNIHKTKESNRTQDRPPTPKRQASTLEASTKSSRYVAKLEDFQLIRVLGKGCAGKVLLVKHAQTHTIHAMKAISKRSVLTHDELKHTMTEVSILRRIAVQEPHNRFVSKLHCSFTDQENFYFVMEFYPGGDLATQMEIYGILGDHRTRFYASDITQGLEDLHRHGIIVRDLKPENILLNAKGHAVLADFGLSKEFAYRGDPKPIHVVTYPGQKELPVWAGKGAGSYRPQASGGGKLVVDKAYSFVGTSEYLSPEVVKRGDYSYAVDWWALGCIVFEGLMGRVPFRKRDEDPPMVLWNKILYDPWDDCFRDPKLAKYALDQTTHKFIDSLLQKDPMRRITEPYVKQHEYFSMIDWGTVQRGEYQDPHGLHIHPTSEYNTRYFPKLCLQEDPSVDMSTHDLRDYEVGGGKRTPMNDNALYKLELVRYRKELEGFAWSRDWDTMWDGEEESEMESGVESFIEESVIEQGREVWDEAGGLIDRPADHEGDAFNQNLTPATSGGLSVPVDYPDPSKSKSLLVPPTSSPENTPVAPMLSLPSITSEFDAATSSPAATFGLPTPAAEGLFNPILSAQPKDNEENGQDEVKTPFVELALKQLESQGIEISIAKKDKTQMVKDAEVMSEEPIIAQAKQLEDLSTDMKSTTGIQDDFKAMNITEKLPSPFKSLSSLASSNEELIPLVPPPPVPTSVIPPPVPAQPVSILDSFPSQTMTNPHPLSTAVFDTLNLPTDLPLSGLSVSDVISVPSLRAGHASPRLLRRHFHREGEISIPLARLSVELHGTITRLEDEGWEELATNKEDIPTAPNCGPGAPYFFRSFTGLRRKPSTLVSSSLRRQYGPDSDTSSRGSSVSPTKSGRRDLKERKLFGGRRSIESTKRLLRSLKTFPRLKSLTQEGGKDKTRVAGPVSPLLSLPSSAPHRSNLRVSSEGNSSDVLGRKAQMTSSDNARRHTESGWIDRHLKRTKPSSAPALQSRVISSSTSNTGNRSDSDEQRGAVSMAEGQVTAPRLELEESKPVVWGF
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 509
B YPD 30°C, concentration: 5x10^7 cells/ml 606
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 653
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 724
E YPD 37°C, concentration: 5x10^7 cells/ml 1170
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 660
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_02389 5' flanking region primer 1TACCTGCCGACAAATGAC
L2CNAG_02389 5' flanking region primer 2TCACTGGCCGTCGTTTTACACATAGCGGCTGCTTTTC
R1CNAG_02389 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGGGGGTTCTAAAAGACG
R2CNAG_02389 3' flanking region primer 2ACCATCATCTCTGCGTTG
SOCNAG_02389 diagnostic screening primer, pairing with B79AACCGCAAGTAGGGCATAC
POCNAG_02389 Southern blot probe primerTGAGCAAAAAAGGCGAGC
STMNAT#242 STM primerGTAGCGATAGGGGTGTCGCTTTAG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_02389T0
Mating Data
Growth phenotype for CNAG_02389T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_02389T0
Urease
Urease phenotype for CNAG_02389T0
Melanin
Melanin phenotype for CNAG_02389T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_02389T0
Oxidative Stress
Oxidative stress phenotype for CNAG_02389T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_02389T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_02389T0
ER Stress
ER stress phenotype for CNAG_02389T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_02389T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_02389T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_02389T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_02389T0